Figure 4
From: The Role of Autophagy in Critical Illness-induced Liver Damage
Effect of hepatic autophagy inactivation on markers of the hepatic UPR in critically ill mice. The ratio of hepatic p-eIF2alpha over eIF2alpha protein expression (a), hepatic mRNA expression of atf4 (b), xbp1s (c), hspa5 (d), dnajb9 (e), creb3l3 (f), calr (g) and pdia4 (h) are shown for healthy pair-fed WT mice (white box plots), healthy pair-fed KO mice (dotted box plots), critically ill WT mice (gray box plots) and critically ill KO mice (hatched box plots) at day 1 and day 3. Box plots depict medians with interquartile ranges (IQR) and whiskers are drawn to the furthest point within 1.5 × IQR from the box. n = 10–16 per group. p-eIF2alpha: phosphorylated eukaryotic initiation factor 2 alpha; eIF2alpha: eukaryotic initiation factor 2 alpha; atf4: activating transcription factor 4; xbp1s: spliced form of x-box binding protein 1; hspa5: heat shock protein family A member 5; dnajb9: dnaJ heat shock protein family member B9; creb3l3: cAMP responsive element binding protein 3-like 3; calr: calreticulin; pdia4: protein disulfide isomerase family A member 4. KO: Mx1-Cre + Atg7 F/F mouse; WT: Mx1-Cre − Atg7 F/F mouse.
