Figure 4

Enzalutamide is toxic to casper zebrafish embryos at high doses by inducing bradycardia. (A) Percentage survival at 72 hpf of casper embryos treated with 10 µM (N = 81 embryos), 13 µM (N = 82 embryos) or 16 µM (N = 74 embryos) of enzalutamide (ENZ) for a total of 4 days. (B) Enzalutamide significantly decreases embryo heart rate (beats per minute – BPM) at 10 µM and 13 µM compared to vehicle control treated embryos, in groups of 30–50 embryos per replicate. Error Bars = Mean ± SEM (N = 6); *P < 0.05, **P < 0.01 for significant differences in heart rate between groups of fish were determined using the Student’s t-test.