Figure 5

The combination of enzalutamide and terfenadine further decrease heart rate and embryo survival. (A) Survival curve of 72 hpf casper embryos treated with vehicle (Control, 2% DMSO) or the indicated concentrations of enzalutamide (ENZ) alone, terfenadine (Terf) alone, and both drugs in combination. Compounds were added to the water of groups of 30–50 embryos and percentage survival over time in days is indicated. (B) Embryonic heart rates decreased significantly when treated with the combination of enzalutamide and terfenadine. Groups of 30–50 embryos were treated with the indicated concentrations of enzalutamide, terfenadine or a combination of both, and the heart rate was determined on the surviving fish after 4 days. Error Bars = Mean ± SEM (N = 6, with the exception of the enzalutamide + tefenadine group for which only 3 animals survived (N = 3)); *P < 0.05, **P < 0.01 for significant differences in heart rate between groups of fish were determined using the Student’s t-test.