Figure 3
From: Norrin protects optic nerve axons from degeneration in a mouse model of glaucoma
Norrin attenuates glaucomatous axonal damage without enhanced expression of Gfap, Edn2, Fgf2 or Bdnf. (A) Immunohistochemistry for GFAP (red) in retinae of two-month-old DBA/2J/Pax6-Norrin mice and DBA/2 littermates. In DBA/2J mice GFAP staining is seen in astrocytes and Müller cell endfeet in the RGC layer and in radial Müller cell processes that extend into the inner plexiform layer. In DBA/2J/Pax6-Norrin littermates the radial staining is more pronounced and reaches into the inner nuclear layer. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Scale bar. 50 µm. Nuclei are labeled with DAPI (blue). (B) Real-time RT-PCR for Gfap, Edn2, Fgf2 and Bdnf in RNA from retinae of eight-week-old DBA/2J/Pax6-Norrin mice and DBA/2J littermates (mean ± SEM).
