Figure 4
From: Norrin protects optic nerve axons from degeneration in a mouse model of glaucoma
Norrin promotes activation of PI3K-Akt signaling via induction of IGF-1. (A) Real-time RT-PCR for Igf1 in retinal RNA of eight-week-old DBA/2J/Pax6-Norrin mice and DBA/2J littermates (mean ± SEM; *p < 0.05). (B) Western blot analysis and relative densitometry for pAKT and AKT in retinal proteins from two-month-old DBA/2J/Pax6-Norrin mice and DBA/2J littermates. For relative densitometry the pAKT/AKT ratio was determined and set to 1 in DBA/2J mice (mean ± SEM; **p < 0.01). (C,D) Immunohistochemistry for pAKT (green) in retinae from DBA/2J and DBA/2J/Pax6-Norrin mice at the age of two (C) and ten (D) months. In both strains immunoreactivity for pAKT is observed in the outer nuclear layer. In DBA/2J mice pAKT immunoreactivity is weak in the ganglion cell layer but intense in two-month-old (C) and a little less pronounced in ten-month-old (D) DBA/2J/Pax6-Norrin littermates. Nuclei are labeled with DAPI (blue). GCL. ganglion cell layer; INL. inner nuclear layer; ONL. outer nuclear layer. Scale bars: 50 µm.
