Figure 1
From: Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
Theory and design of the custom filter cube. (A–C) Images of a human orbitofrontal cortex (OFC) tissue section labeled for somatostatin (SST) mRNA conjugated to Alexa Fluor 488. Single plane image of (A) SST mRNA and (B) lipofuscin autofluorescence. The excitation (ex) and emission (em) filter properties (wavelength/bandwidth) used for image capture are indicated. (C) A merged image of A and B. (D) Schematic illustration of the custom filter cube. (E) Plot showing relative em intensities for Alexa Fluor 488 and for lipofuscin autofluorescence excited by a 488 nm laser. The shaded regions correspond to the ex and em filter settings used for the custom filter cube. (F) Image of the same tissue section as in A-C but captured using the custom filter cube. Arrows indicate lipofuscin autofluorescence (solid arrowheads) and SST mRNA grains (open arrowheads). Bars = 10 μm.
