Table 2 Time and estimated cost analysis for rDNA sequencing from one isolate using conventional Sanger sequencing versus MALDI-TOF-MS biotyping.
DNA sequencing (Sanger) | MALDI-TOF-MS | ||
|---|---|---|---|
Hands-on time | Hands-off time | Hands-on time | Hands-off time |
Process/work time | |||
DNA extraction | Protein extraction | ||
20 min | 0 min | ||
Nested PCR steps | 1 min | 5 min | |
40 min | 5 hr | ||
Cloning steps | MALDI sampling | ||
3–4 hr | 1–2 days | ||
Sequencing reaction | 10 min | 5 min | |
25 min | 4 hr | ||
Sequencing clean up | Data acquisition | ||
30 min | 0 hr | ||
Sanger Sequencing | 5 min | 1 min | |
5 min | 1 hr | ||
Total time | |||
Total time/sample | Total time/sample | ||
5–6 hr | 34–58 hr | 16 min | 11 min |
Approximate cost | |||
(Chemistry and consumables) | |||
Total Price/sample | Total Price/sample | ||
17 US $ with cloning | 3 US $ | ||
9.5 US $ without | |||
Approximate cost | |||
(Instruments) | |||
95000 US $ | 155000 US $ | ||
