Figure 3
From: Phosphoproteomics of cAMP signaling of Bordetella adenylate cyclase toxin in mouse dendritic cells
Changes in phosphorylation status of selected phosphoproteins. Members of mTOR signaling pathway after the toxin/toxoid treatment for (A) 10 minutes or (B) 30 minutes. (C) Phosphorylation status of serine 235 residue of cytoskeletal regulator VASP. In general, 106 BMDCs in 1 mL were exposed for 10 or 30 minutes to 100 ng of CyaA or of the CyaA-AC- toxoid, cellular lyzates were separated by SDS-PAGE and phosphorylation of indicated proteins was probed by Western blotting with specific antibodies. Images representative of at least three biological replicates are shown and quantifications of the Western blots of mTOR signaling pathway members are shown in Supplementary Fig. S6. Cropped images are shown and full-length blots are presented in Supplementary Fig. S7. “Buffer” stands for TUC-treated cells (TUC buffer −50 mM Tris-HCl, 8 M urea, 2 mM CaCl2, pH 8; used for toxin/toxoid dilution).
