Figure 1
Sweeteners induced sleep in starved flies. Flies fed with 150 mM sucrose on day 0 were transferred to agar containing 150 mM sucrose, no sugar, 150 mM sucralose, 300 mM arabinose or 300 mM sorbitol on day 1, and locomotor activity was monitored for 3 d in the 12-h light: dark cycle (LD) condition. (a) Average sleep amounts in 30-min intervals for each group having one of five foods on day 1 and day 2 are plotted (n = 16, 15, 14, 14, 14 flies, respectively). Day and night are depicted by the white and black bars, respectively. (b-e) Summary of the sleep parameters of daytime (ZT 0–12) of day 2. Total sleep amount (b), total activity counts (c), waking activity index (d) and mean sleep bout duration (e) are shown. Waking activity is defined as the total daily activity divided by the length of the daily active period. Data are represented as mean ± s.e.m. Statistical significance between sucrose and each food was calculated using a one-way ANOVA with a Tukey-Kramer HSD post hoc test. *P < 0.05.
