Figure 3
Activation of gustatory sugar receptor expressing neurons in starved flies induced sleep. Flies of indicated genotypes fed with 150 mM sucrose at 22 °C on day 0 are transferred to different foods as in Fig. 1 at 22 °C on day 1, and the temperature was shifted to 29 °C at ZT 0 on day 2 to activate dTrpA1 expressing gustatory neurons. (a and b) Average sleep amounts in 30-min intervals of each group are plotted. (a) Gr43a-GAL4/+ (n = 32, 30, 31) (b) Gr43a-GAL4/UAS-dTrpA1 (n = 32, 30, 32) (c–e) Total sleep amount for control and flies with Gr neuron activation on the first half of nighttime (6 hrs from ZT 12 to ZT 18) on day 2; Gr5a (n = 19, 16, 18, 32, 16, 32), Gr43a (n = 32, 18, 29, 32, 19, 32), Gr64a (n = 20, 13, 19, 27, 15, 29). Data are represented as mean ± s.e.m. Statistical significance between control and flies with Gr expressing neuronal activation was calculated using a one-way ANOVA with a Tukey-Kramer HSD post hoc test. *P < 0.05.
