Figure 1 | Scientific Reports

Figure 1

From: Upregulation of microRNA-4417 and Its Target Genes Contribute to Nickel Chloride-promoted Lung Epithelial Cell Fibrogenesis and Tumorigenesis

Figure 1

Effects of TGF-β receptor inhibition on EMT markers in nickel-treated cells. (a) BEAS-2B (1 × 106 cells/6 cm dish) and (b) A549 (5 × 105 cells/6 cm dish) cells were treated with NiCl2 (0.5 and 1 mM, respectively) or TGF-β (10 ng/ml) with or without SB525334 (10 μM) for 72 h and the protein levels were analyzed on Western blot. β-actin was used as the internal control. The relative ratios of Fibronectin/β-actin, E-cadherin/β-actin, HIF-1α/β-actin, TAB2/β-actin, ENOSF1/β-actin, and RAB6A/β-actin are shown. (c) BEAS-2B cells (1 × 106 cells/6 cm dish) were treated with NiCl2 (0.5 mM) for 15 min, 30 min, 1 h and 24 h or TGF-β (10 ng/ml, positive control) for 30 min. The protein levels were analyzed on Western blot. β-actin was used as the internal control. The relative ratios of p-Smad2/Smad2, p-Smad3/Smad3 and Smad4/β-actin are shown.

Back to article page