Figure 2
NiCl2 correlates with miRNA expression patterns. (a) BEAS-2B cells (1 × 106 cells/6 cm dish) were treated with NiCl2 (0.5 mM) for 48 h. Heat maps and cluster analysis of the 46 miRNA differentially expressed between control and 0.5 mM NiCl2-treated BEAS-2B cells. Up- and down-regulated genes are represented in red and green, respectively. (b) Ten of the 46 miRNAs identified on microarray were corroborated with qRT-PCR data. BEAS-2B cells were treated with NiCl2 (0, 0.5 mM) for 48 h. The log2 of the ratios of expression levels are shown. (c) Seven miRNAs identified as significantly different between Ni-treated A549 cells and controls on microarray study were evaluated on qRT-PCR. All values have been normalized to the level of RNU6B and are the averages of three independent readings. *p < 0.05 and **P < 0.01 (d) BEAS-2B cells (1 × 106 cells/6 cm dish) were transfected with microOFFTM miR-4417 inhibitor (100 and 200 nM) or scrambled miRNA inhibitor (scramble inhibitor, 200 nM) followed by exposure to NiCl2 (0 and 0.25 mM) for 48 h. Quantitative real-time PCR analysis was used to detect miR-4417 expression. All values have been normalized to the level of RNU6B and are the averages of three independent readings. *p < 0.05; ##p < 0.01.
