Figure 6
Photomicrographs of representative lung cancer formation in nickel-treated mice. After oral administration of 0 and 20 mg NiCl2/kg/day for 15 days, the mice were inoculated with or without BEAS-2B cells (1.5 × 106 cells/mouse) via intravenous route. The mice were continually exposed to (a) ddH2O or (b) NiCl2 for 60 days and sacrificed at 75 days. The tumor development in lung tissues were investigated by H&E stain. Photographed at 100× magnification. Black arrow points to the tumor cells and black triangles to cell fibrogenesis. Lung tissue sections from ddH2O group (c and d) and NiCl2 group (e and f) were analyzed by immunohistochemistry using a human cytokeratin 19 antibody. The compositional range enclosed by the quadrilateral of (c) and (e) are enlarged in (d) and (f), respectively. Photomicrographs of IHC for human cytokeratin 19 in the lung tissue of the nude (magnification: left, 40× and right, 100×). The black arrows point to the tumor cells. (g) Model depicting the mechanisms of Ni-induced fibronectin.
