Figure 3
Disruption of WRKY43 decreases germination on ABA but increases tolerance to NaCl, cold and mannitol. (A) Germination (radicle emergence) of Ler-0 and wrky43-1 after-ripened seeds after 10 days incubation on increasing ABA concentrations. Data were normalized and fitted (log(inhibitor) vs. normalized response, Variable slope) to calculate IC50 values (mean ± SE, n = 3). (B) Germination (radicle emergence) of after-ripened seeds after 10 days incubation on 4 µM ABA-containing 0.5 MS medium (mean ± SE, n = 3). Asterisks indicate significant differences using an unpaired t-test (p < 0.05). (C) Germination (radicle emergence) of Ler-0 and wrky43-1 after-ripened seeds after 3 days incubation on increasing NaCl concentrations. Data were normalized and fitted (log(inhibitor) vs. normalized response, Variable slope) to calculate IC50 values (mean ± SE, n = 3). Asterisks indicate significant differences using an unpaired t-test (p < 0.05). (D) Germination (radicle emergence) of after-ripened seeds after 3 days incubation on 150 mM NaCl-containing 0.5 MS medium (mean ± SE, n = 3). Asterisks indicate significant differences using an unpaired t-test (p < 0.05). (E) Germination (radicle emergence) of Ler-0 and wrky43-1 after-ripened seeds after 4 days incubation on 0.5 MS medium at decreasing temperatures from 28 °C to 10 °C (mean ± SE, n = 3). (F) Germination (radicle emergence) of after-ripened seeds after 4 days incubation on 0.5 MS medium at 10 °C (mean ± SE, n = 3). (G) Germination (radicle emergence) of Ler-0 and wrky43-1 after-ripened seeds after 3 days incubation on increasing mannitol concentrations. Data were normalized and fitted (log(inhibitor) vs. normalized response, Variable slope) to calculate IC50 values (mean ± SE, n = 3). Asterisks indicate significant differences using an unpaired t-test (p < 0.05).
