Figure 4
Disruption of WRKY43 increases polyunsaturated fatty acid content in TAGs and phospholipids. (A) Total fatty acid content of dry seeds. Data are shown as box plots with Tukey whiskers, with plus signs indicating means. (B) Triacylglycerol (TAG) fatty acid composition of dry seeds. Statistical analysis was performed with one-way ANOVA (p < 0.05) and Bonferroni posttest; asterisks indicate significant differences compared with the wild type. (C) %18:1/%18:2 desaturation rate (FAD2 activity) and %18:2/%18:3 desaturation rate (FAD3 activity). Data are shown as box plots with Tukey whiskers, with plus signs indicating means. Statistical analysis was performed with one-way ANOVA (p < 0.05) and Bonferroni posttest; asterisks indicate significant differences compared with the wild-type Ler-0. (D) Fatty acid composition of phosphatidylcholine (PC). (E) Fatty acid composition of phosphatidylinositol (PI) and phosphatidylserine (PS). (F) Fatty acid composition of phosphatidylethanolamine (PE). Fatty acid composition of phospholipids in 8 DAF old seeds of Ler-0 and wrky43-1 mutant. Data are shown as bar charts. Statistical analysis was performed with one-way ANOVA (p < 0.05) and Bonferroni posttest; asterisks indicate significant differences compared with Ler-0 wild type.
