Figure 3
From: Oligomerization and ATP stimulate condensin-mediated DNA compaction
Single molecule magnetic tweezers compaction traces for multimer and monomer condensin with and without ATP. (A) Compaction by multimeric condensin with ATP. The readout of each experiment is the length of the DNA tether. Under 0.45 pN force, the naked DNA is extended to ≈2.4 μm. When multimeric condensin is added in the presence of ATP (“flow-through”), the DNA tether is compacted by ≈70%. (B) Compaction by multimeric condensin without ATP. Similar to Fig. 3A but without ATP, the DNA tether is compacted by condensin but to a final compaction percentage of ≈45%. (C) Compaction by monomeric condensin with ATP. Similar to Fig. 3A but with monomeric condensin instead of multimeric, the DNA tether is compacted by condensin to a final compaction percentage of ≈45%. (D) Compaction by monomeric condensin without ATP. Similar to Fig. 3B but with monomeric condensin instead of multimeric, the DNA tether is compacted by condensin to a final compaction percentage of ≈45%.
