Figure 4
From: Freeform micropatterning of living cells into cell culture medium using direct inkjet printing

Demonstration of various cell patterns that were directly printed into culture medium. (a) Printed cell migration assay model with 1-mm and 0.5-mm gaps. Images were captured at 2 hours and 48 hours after printing. Scale bar: 500 µm. (b–e) Fluorescent images of red-, green-, and blue-labeled cell patterns. Cells were pre-labeled and printed in patterns. Scale bar: 1 mm. (f) Printed zigzag pattern using a heterotypic co-culture model of NIH3T3 and HEK293A cells. Scale bar: 1 mm.