Figure 3 | Scientific Reports

Figure 3

From: Characterization of an industry-grade CMOS camera well suited for single molecule localization microscopy – high performance super-resolution at low cost

Figure 3

The CMOS camera allows for dSTORM imaging over a wide range of frame rates and fields-of-view. (a) Dependent on the ROI, imaging with the CMOS camera can be performed at different maximum frame rates. The green boxes visualize the relations between the listed ROI dimensions. (b,d) A microtubule image reconstructed from 10,000 frames recorded at a rate of 894 fps. (c,f) Imaging a similar sample at 40 fps. (e,g) Line profiles along straight microtubules reveal the hollow microtubule structure both for high and low frame rate imaging. Scale bars on (b,c) 1 µm. Scale bars on (d,f) 100 nm.

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