Figure 1

Positive PMP2 ICC. HEK293 cells were transfected with a mammalian expression vector encoding PMP2 with the use of Lipofectamine 2000 (Invitrogen, CA, USA) and ICC was performed as described in Supplementary Table S3. Patient’s 22 sera positivity can be appreciated in green, commercial antibody staining in red and a merged picture of both can be found above with nuclei stained in blue.