Figure 2
From: Thyroid hormone inhibits growth of hepatoma cells through induction of miR-214
miR-214 inhibits the proliferation of HCC cell lines. Huh7 cell lines stably expressing miR-214 were established by transfection with the miR-214 plasmid. (a) The expression levels of miR-214 were measured by qRT-PCR and the vector pcDNA6.2 was used as a control, respectively. (b) The Huh7 hepatoma cell line proliferation capacity was measured by the total cell numbers. (c) Colony assay in the Huh7 cells. (d) The stable knockdown of miR-214 in the SK-Hep1 cell lines was established by infection with the lentivirus miRZip-miR-214 (Anti-miR-214). The expression levels of anti-miR-214 were measured by qRT-PCR. U6 was used as a loading control. (e) The SK-Hep1 hepatoma cell lines proliferation capacity was measured. (f) The colony assay in SK-Hep1. The data are presented as the means ± s.d. of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001 vs. control vector).
