Figure 7
From: Efficacy of an Adenoviral Vectored Multivalent Centralized Influenza Vaccine
Micro-neutralization and Memory T Cell. Sera from vaccinated mice was serially diluted and incubated with 50 TCID50 of each indicated influenza virus for 1 h. 2.5 × 105 MDCK cells were added to the wells and the plates were incubated for 5 days. 50 µl of 0.5% chicken RBC solution was added to all wells and incubated at room temperature for 1 h before reading. The neutralization titer was determined as the highest dilution of sera to prevent agglutination (A). Splenocytes from the vaccinated mice were collected and pooled. The splenocytes were stimulated with peptides representing the 4 H3 proteins, H3-con, TX/77, Aichi/68 and MS/85. The peptides consisted of 17-mers overlapping by 12 amino acids. The assays were run in duplicate. Interferon gamma secreting cells were detected using AN18 and R4-6A2 antibodies and the total spot forming cells (SFC)/106 splenocytes are shown (B).
