Figure 1 | Scientific Reports

Figure 1

From: Decoy Wnt receptor (sLRP6E1E2)-expressing adenovirus induces anti-fibrotic effect via inhibition of Wnt and TGF-β signaling

Figure 1

Reduced β-catenin/TCF transcriptional activity by decoy Wnt receptor sLRP6E1E2. TCF/LEF luciferase reporter assay was performed in human dermal fibroblasts (HDFs) and keloid fibroblasts (KFs) to characterize the sLRP6E1E2 effects on the Wnt3a/β-catenin signaling. HDFs and KFs were initially transfected with TOPFLASH (containing wild-type TCF binding sites) plasmid, and then transduced with dE1-k35/LacZ or dE1-k35/sLRP6E1E2 (50 MOI) with canonical Wingless type (Wnt)3a (100 ng/mL) and transforming growth factor (TGF)-β1 (20 ng/mL). Transduction with dE1-k35/sLRP6E1E2 reduced β-catenin/TCF transcriptional activity to a basal level, indicating that sLRP6E1E2 can inhibit the transcription of Wnt target genes. The data are representatives of three independent experiments (**p < 0.01).

Back to article page