Figure 2
Effect of sLRP6E1E2-expressing adenovirus (Ad) on the mRNA levels of type-I and -III collagen. Quantitative mRNA levels of type-I and -III collagen in HDFs and KFs were assessed by qRT-PCR after transduction with dE1-k35/LacZ or dE1-k35/sLRP6E1E2 (20, 50, and 100 MOI) in the presence of TGF-β1 (20 ng/mL). Both type-I (a) and type-III (b) collagen mRNA levels in HDFs treated with dE1-k35/sLRP6E1E2 were significantly lower than those in dE1-k35/LacZ-treated HDFs (**p < 0.01). Also, in KFs, type-I (c) and type-III (d) collagen mRNA levels treated with various amouns of dE1-k35/sLRP6E1E2 were significantly lower than those in dE1-k35/LacZ-treated KFs (**p < 0.01). Data are expressed as mean ± SD of a representative experiment from 3 independent experiments performed with triplicate samples.
