Figure 3
Effect of sLRP6E1E2-expressing Ad on TGF-β1 and Wnt3a/β-catenin signaling pathway. (a) Immunoblotting analysis was performed to analyze the expression levels of LRP6, β-catenin, pGSK-3β, and Smad 2/3 complex in HDFs after transduction with either dE1-k35/LacZ or dE1-k35/sLRP6E1E2 (50 MOI) in the presence of Wnt3a. (b and c) Secreted TGF-β1 protein was measured by ELISA. HDFs and KFs were treated with TGF-β1 to induce fibrosis, and then transduced with dE1-k35/sLRP6E1E2 (50 MOI). Following transduction with dE1-k35/sLRP6E1E2, TGF-β1-activated HDFs (b), KFs, and TGF-β1-activated KFs (c) exhibited significantly reduced TGF-β1 expression (**p < 0.01). Data are expressed as mean ± SD of a representative expreriment from 3 independent experiments performed with triplicate samples.
