Figure 5
Decreased expression of Wnt3a, TGF-β1, and major ECM components in keloid tissue explants by sLRP6E1E2-expressing Ad. (a) Immunohistochemical staining demonstrates that the expression levels of both Wnt3a and TGF-β1 are decreased in keloid tissue explants treated with either JW 55 (10 mM) or dE1-k35/sLRP6E1E2 (50 MOI), compared to those in dE1-k35/LacZ-transduced (50 MOI) keloid tissue explants. Original magnification: ×1000 (b) Results from immunohistochemical analysis of Wnt3a and TGF-β1 were semi-quantitatively analyzed by MetaMorph® image analysis software (**p < 0.01, *p < 0.05) (c) Masson’s trichrome or Picrosirius red staining was performed to assess collagen intensity and deposition in keloid tissue explants. Transduction with dEl-k35/sLRP6E1E2 (50 MOI) markedly decreased collagen intensity and deposition than those transduced with dEl-k35/LacZ (50 MOI). Original magnification: ×400 (d) Immunohistochemical staining for type-I and -III collagen, elastin, and fibronectin in keloid tissue explants. Keloid tissue explants treated with JW 55 or dE1-k35/sLRP6E1E2 exhibited markedly attenuated expression level of major ECM components in comparison to dE1-k35/LacZ-transduced keloid tissue explants. Original magnification: ×1000. (e) Results from immunohistochemical analysis of major ECM components were semi-quantitatively analyzed by MetaMorph® image analysis software (**p < 0.01). The data are representatives of three independent experiments.
