Figure 3
From: Female mice lacking Pald1 exhibit endothelial cell apoptosis and emphysema
Pald1 −/− mice show reduced complexity of the lung tissue. (a,b) Dark field images of distal airspace from 4 weeks old female Pald1 wild type (a) and knock-out animals (b), suggest a general airspace enlargement in the Pald1 knock-out lungs. Scale bars = 200 µm. (c) Quantification of interseptal alveolar distance using Mean Linear Intercept (MLI) of hematoxylin-eosin stained lung tissue at P5 (n = 3), 4 (n = 5) and 19 weeks (n = 4) shows increased air spaces in female knock-out animals at all stages, but was particularly increased at 4 weeks. ANOVA per age group: P5 p < 0.0001; 4 weeks p = 0.0002; 19 weeks p = 0.0004. Pald1 −/− was compared to Pald1 +/+ of the same sex within each age group. (d) MLI of Pald1 wild type, heterozygous and knock-out female mice at the indicated ages. ANOVA per age group: 4 weeks p = 0.0001, 19 weeks p = 0.0007. Pald1 −/− and Pald1 +/− were compared to Pald1 +/+ within each age group. Error bars: SD, *p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001.
