Figure 7
Protein levels of mitochondrial respiratory chain complexes and Tfam in atria of OSA dogs and metoprolol-treated dogs. (A) Transmission electron microscopy images in dogs from the Sham group, OSA group and MET group, direct magnification: 8000 × (scale bar: 2 μm). n = 6 each group. (B) Representative immunoblots of Tfam in atrial tissue and statistical results for Tfam. (C) Representative immunoblots of HIF-1α in atrial tissue and statistical results for Tfam. (D) Western blot results for NDUFB8, SDHB, UQCRC2, MTCO1, and ATP5A protein expression and the quantification of NDUFB8, SDHB, UQCRC2, MTCO1, and ATP5A protein levels. NDUFB8 indicates a subunit of nicotinamide-adenine dinucleotide dehydrogenase (complex I); SDHB, 30-kDa subunit of succinate dehydrogenase (complex II); UQCRC2, core protein 2 of ubiquinol-cytochrome c reductase complex (complex III); MTCO1, subunit I of cytochrome c oxidase (complex IV); ATP5A, a subunit of F 0 F 1-ATP synthase (complex V). The expression levels of these proteins were normalized to the level of GAPDH. **p < 0.01, ***p < 0.001 vs Sham group, # p < 0.05, ## p < 0.01, ### p < 0.001 vs OSA group, n = 5 per group. (E) The atrial tissue concentration of adenosine triphosphate (ATP) was measured using an ATP assay. The data are presented as the mean ± standard. **p < 0.01 vs Sham group, n = 4 per group.
