Figure 2
From: Elimination of a signal sequence-uncleaved form of defective HLA protein through BAG6
Proteasome is responsible for the degradation of the SS-uncleaved form of HLA-A. (a) T7-tagged mislocalized species of HLA-A*6802 protein (MLP-HLA) are degraded in a proteasome-dependent manner. HeLa cells expressing T7-tagged HLA-A*6802 were treated with 2 μM bortezomib, 10 μM epoxomicin, 10 μM Leupeptin and 10 μM MG-132. At 2 h after inhibitor treatments, the cells were lysed and the accumulation of HLA-A*6802 proteins was examined by either anti-T7 or anti-Flag antibodies. Mock indicates empty vector transfection. DMSO solvent was used as a negative control for inhibitor treatments. Actin was used as a loading control. (b) The graph quantitatively displays the data of the above blot signals as mean ± S.D. calculated from at least 3 independent experiments. *P < 0.05 compared with DMSO control. Full-length images of blots are presented in Supplementary Fig. S2.
