Figure 3
From: Elimination of a signal sequence-uncleaved form of defective HLA protein through BAG6
BAG6 is essential for the elimination of the SS-uncleaved form of HLA-A. (a) HeLa cells were transfected with two distinct siRNA duplexes for bag6 (siRNA#1 and #2) or control siRNA (5 nM each). At 48 h after siRNA transfection, T7-tagged HLA-A*6802 was expressed in the cells. At 24 h after HLA-A*6802 transfection, the cells were harvested and lysates were probed with anti-T7- and anti-Flag-antibodies as in Fig. 2a. The band indicated by an asterisk is non-specific. The BAG6 blot indicates the efficient depletion of BAG6 protein by its siRNA. Actin was used as a loading control. (b) The graph indicates the quantified data of the anti-T7 signals in the above blot as mean ± S.D. calculated from at least 3 independent experiments. n = 4 for bag6 siRNA#1. n = 3 cells for bag6 siRNA#2. *P < 0.05 compared with control siRNA cells. (c) HeLa cells were transfected with siRNA duplexes for bag6 (siRNA#1), ubqln4 (siRNA #2) or control siRNA as in (a). Full-length images of blots are presented in Supplementary Fig. S3.
