Figure 1
Effect of AMP induced HMS on Tc. (a) B6 mice were administered intraperitoneally 0.5 mg/g b.w. of AMP with or without indicated treatments and immediately transferred to a Ta of 15 °C for a period of 6 hours. Thereafter, mice were brought back to room temperature (25 °C) and allowed to recover. The Tc was monitored on an hourly basis using RET3 rectal probe (two way ANOVA after Boneferoni post-test: F = 384.7and P < 0.0001 for interaction, F = 692.5 and P < 0.0001 for time, F = 1629.0 and P < 0.0001 for treatment, ***p < 0.001 for all AMP treated group at 150 C (1–6 h) and at 260 C along with CAF (1–2 h) when compared to control + 15 °C). Changes in the expression of Hif1α in frontal cortex (b) and femur (c) 6 hours after indicated treatments. (d). Central activation of adenosine receptor in AMP mediated HMS. Vehicle or AMP in the presence or absence of caffeine was administered directly into frontal cortex (intracerebroventricular region) and changes in Tc was measured on an hourly basis for the duration of the experiment. Each value is a mean ± SEM (n = 3–6 mice/group) and comparisons were made between indicated groups. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant. Caf represents caffeine.
