Figure 7 | Scientific Reports

Figure 7

From: Monocytes from patients with Primary Ciliary Dyskinesia show enhanced inflammatory properties and produce higher levels of pro-inflammatory cytokines

Figure 7

PBMCs from PCD patients produce more pro-inflammatory chemokines (CCL3, CCL18 and CCL22) compared to PBMCs from pediatric healthy controls. PBMCs from PCD patients (PCD), age-matched healthy pediatric controls (Ped CO) and healthy adult controls (Ad CO) were cultured and stimulated with LPS (5 µg/ml), dsRNA (PIC; 10 µg/ml), conA (10 µg/ml), PGN (1 µg/ml) or IL-1β (100 ng/ml) at 37 °C for 24 h. By sandwich ELISAs, (a) CCL3, (b) CCL5, (c) CCL18 and (d) CCL22 levels were measured in the supernatants. The cytokine levels are expressed relative to the chemokine levels produced by PBMCs from the reference Ad CO (equal to 100%, not shown). (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; Mann Whitney U-test).

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