Figure 2 | Scientific Reports

Figure 2

From: Detection of subclonal L1 transductions in colorectal cancer by long-distance inverse-PCR and Nanopore sequencing

Figure 2The alternative text for this image may have been generated using AI.

Agarose gel image of the LDI-PCR products. LDI-PCR using three different inverse primer pairs and restriction enzymes on two colorectal tumours (c985T and c368T) and their matching normal (c985N and c368N) DNA samples. The “native” PCR product of expected size and several additional tumour-specific products representing different putative 3′ transduction targets were detected. (Sizes corresponding to native LDI-PCR products: PstI~6.3 kb, NsiI~10.2 kb, SacI~5.6 kb.) Digested/self-ligated blood genomic DNA sample of an unrelated individual was run in the lane labelled “b” and PCR without any template in “−”.

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