Figure 5 | Scientific Reports

Figure 5

From: Detection of subclonal L1 transductions in colorectal cancer by long-distance inverse-PCR and Nanopore sequencing

Figure 5The alternative text for this image may have been generated using AI.

Extrapolated mechanism of L1 insertion at (a) GRID2 locus and (b) CNTNAP2 locus In both (a) and (b) (i) shows locations of staggered double-stranded cleavage in the target region with PolyT primer in the 3′ overhang and an internal primer (when used) in the 5′ overhang (b). (ii) Extrapolated L1 mRNA with the TTC28 3′ unique tag. Regions complementary to the polyT primer and internal primer at target site is highlighted by the same colour scheme. (iii) Schematic representation of the TPRT (a) and twin-priming (b) mechanism of TTC28 specific 3′ transduction in the GRID2 (a) and CNTNAP2 (b) locus. Figures are not drawn to scale.

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