Figure 4
From: Identification of novel cis-regulatory elements of Eya1 in Xenopus laevis using BAC recombineering
GFP reporter expression driven by BAC15. Embryos of Xenopus laevis at neural plate (A–D: dorsal view, anterior to the left) and tailbud stages (A’–D’: lateral view, anterior to the left) after injection with BAC15 reporter constructs at the one-cell stage. A,A’,B,B’: GFP expression after injection of full length BAC15 reporter (BAC15-FL) in living Xenopus embryos (A,A’) or as revealed by GFP in situ hybridization (B,B’). C,C’: Absence of GFP expression domains after injection of BAC15-P2D2 deletion construct. D,D’: GFP expression domains after injection of BAC15-P2D4 deletion construct recapitulate those observed after BAC15-FL injection. Insert in D’ shows somitic expression in trunk region of a different embryo (tail end to the right). Abbreviations: epi: epidermis; np: neural plate; pp: pharyngeal pouches; PPR: preplacodal region; som: somites.
