Table 1 Expression domains mediated by BAC15.

From: Identification of novel cis-regulatory elements of Eya1 in Xenopus laevis using BAC recombineering

Expression Constructs

Neural plate stage embryos (14–18)

Tailbud stage embryos (23–40)

Epidermis % (n)

PPE % (n)

Neural plate % (n)

Epidermis % (n)

Head ectoderm/ placodes % (n)

Somites % (n)

Pharyngeal pouches/gut % (n)

BAC15-control1

0 (53)

0 (53)

0 (53)

0 (8)

0 (8)

0 (8)

0 (8)

BAC15-FL2

81 (124)

75 (124)

81 (124)

74 (114)

74 (114)

71 (114)

30 (114)

BAC15-P1D13

nd

nd

nd

nd

nd

nd

nd

BAC15-P1D23

78 (49)

73 (49)

78 (49)

76 (129)

62 (129)

75 (129)

33 (129)

BAC15-P1D33

nd

nd

nd

nd

nd

nd

nd

BAC15-P1D43

79 (43)

72 (43)

79 (43)

90 (10)

90 (10)

90 (10)

60 (10)

BAC15-P2D13

0 (46)

0 (46)

0 (46)

0 (64)

0 (64)

3 (64)

0 (64)

BAC15-P2D23

0 (44)

0 (44)

0 (44)

0 (61)

0 (61)

0 (61)

0 (61)

BAC15-P2D33

97 (37)

97 (37)

89 (37)

50 (4)

50 (4)

25 (4)

0 (4)

BAC15-P2D43

100 (29)

97 (29)

93 (29)

67 (6)

67 (6)

50 (6)

0 (6)

  1. n: number of embryos analyzed; nd: not determined.
  2. 1Empty BAC15 (no insert).
  3. 2BAC15 full length (FL).
  4. 3BAC15 deletion constructs (see Fig. 3).