Table 2 Expression domains mediated by BAC16.

From: Identification of novel cis-regulatory elements of Eya1 in Xenopus laevis using BAC recombineering

Expression Constructs

Neural plate stage embryos (14–18)

Tailbud stage embryos (23–40)

Epidermis % (n)

PPE % (n)

Neural plate % (n)

Epidermis % (n)

Head ectoderm/ placodes % (n)

Somites % (n)

Pharyngeal pouches % (n)

BAC16-control1

0 (59)

0 (59)

0 (59)

0 (8)

0 (8)

0 (8)

0 (8)

BAC16-FL2

71 (234)

67 (234)

68 (234)

60 (148)

76 (148)

35 (148)

0 (50)

BAC16-P1D13

85 (54)

83 (54)

78 (54)

78 (124)

69 (124)

23 (124)

0 (82)

BAC16-P1D23

nd

nd

nd

nd

nd

nd

nd

BAC16-P1D33

55 (29)

41 (29)

41 (29)

65 (69)

46 (69)

26 (69)

0 (69)

BAC16-P1D43

72 (152)

68 (152)

71 (152)

73 (146)

60 (146)

50 (146)

0 (146)

BAC16-P2D13

7 (41)

0 (41)

0 (41)

0 (51)

0 (51)

2 (51)

0 (51)

BAC16-P2D23

nd

nd

nd

nd

nd

nd

nd

BAC16-P2D33

0 (48)

0 (48)

0 (48)

0 (73)

0 (73)

0 (73)

0 (73)

BAC16-P2D43

69 (36)

50 (36)

44 (36)

61 (70)

49 (70)

33 (70)

16 (70)

  1. n: number of embryos analyzed; nd: not determined.
  2. 1Empty BAC16 (no insert).
  3. 2BAC16 full length (FL).
  4. 3BAC16 deletion constructs (see Fig. 3).