Figure 1
From: Vascular endothelial growth factor signaling requires glycine to promote angiogenesis
Glycine was required in the VEGF signals. (a) HUVECs cultured in the medium with or without amino acids were treated with 20 ng/ml VEGF for 24 hours. Immunoblots of P-S6 and S6 were shown. Membranes were reprobed for ß-actin as loading control. Gray values were analyzed by the Image J software and presented in the graph (n = 3). (b) Intracellular content of alanine(Ala), arginine(Arg), asparagine(Asn), aspartic acid(Asp), cysteine(Cys), γ-aminobutyric acid(Gaba), glutamine(Gln), glutamic acid(Glu), glycine(Gly), histidine (His), isoleucine(Ile), leucine(Leu), lysine 37, methionine(Met), phenylalanine(Phe), proline(Pro), serine(Ser), threonine(Thr), tryptophan(Trp), tyrosine(Tyr) and valine(Val) in control and 20 ng/ml VEGF-treated ECs for 24 h. LC-MS was used to detect relative intracellular quantities of amino acids in ECs in the absence and presence of VEGF (n = 3). (c) Immunoblot of total S6 and P-S6 in untreated and different amino acids-treated ECs. The concentration of amino acids: glycine(140 uM), leu(1.33 mM), tyro(1 mM), ala(1 mM), ser(1 mM). The treatment time was 5 h. Quantitative analysis was presented in the graph (n = 3).
