Figure 3
From: Safety of Nonporous Silica Nanoparticles in Human Corneal Endothelial Cells

LDH assay. LDH released from HCECs was measured at 24 h (A to C) and 48 h (D to F) of incubation of various concentrations of 50, 100, 150 nm-sized SiNPs. LDH decreased significantly with SiNPs of 100 nm at a 75 μg/mL concentration after 24 h of incubation (B) and with SiNPs (10–100 μg/mL concentration) of 50 nm after 48 h of incubation. Triplicates of each treatment group were used in each independent experiment. Values are the mean ± SEM from three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001).