Figure 3 | Scientific Reports

Figure 3

From: Identification of a peptide-peptide binding motif in the coating of nab-paclitaxel nanoparticles with clinical antibodies: bevacizumab, rituximab, and trastuzumab

Figure 3

Bevacizumab Heavy Chain Peptide Library Kinetic Binding Parameters Determined by Biacore-SPR: A peptide library of bevacizumab was screened via Biacore SPR against HSA Peptide 40. The bevacizumab library was run over HSA Peptide 40 immobilized to a CM5 chip via amine coupling and vice versa. All peptides were run in the pM-uM concentration ranges. (a) Sensograms of HSA Peptide 40 run over immobilized Bevaciuzmab peptides 11 and 12 (120 second association period, 180 second dissociation period). Short gaps in binding curves at transition times are from removal of solvent spikes during analysis. Binding kinetics were determined via Biacore Evaluation Software analysis of SPR sensograms. (b) Location and structure of amino acids 90–122 on the heavy chain variable domain from a crystal structure of bevacizumab in complex with vascular endothelial growth factor (VEGF). Structure, PDB 1BJ1, was visualized using UCSF Chimera.

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