Figure 2 | Scientific Reports

Figure 2

From: Site Specific Modification of Adeno-Associated Virus Enables Both Fluorescent Imaging of Viral Particles and Characterization of the Capsid Interactome

Figure 2

Viral particles are detected and distribute into distinct patterns in vivo (A) Confocal z-stack projections of images taken from coronal sections (40 µm) of adult mice (n = 3 mice were analyzed for both AAV9 and AAV9-138) show a clear fluorescent signal from the viral particles in the cortex (CTX), corpus callosum (CC) and caudate putamen (CP) two hours after intravenous delivery, Scale bar, 50 µm (B) Capsid proteins were distributed into three distinct patterns after two hours: (1) Away from the nucleus (25.7% ± 1.6%); (2) Perinuclear (66.1% ± 6.6%); (3) Intranuclear (2.1% ± 0.5%); 400 capsid puncta counted. Hoechst 33342 counterstained nuclei stained blue; Scale bar, 10 µm.

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