Figure 5 | Scientific Reports

Figure 5

From: Site Specific Modification of Adeno-Associated Virus Enables Both Fluorescent Imaging of Viral Particles and Characterization of the Capsid Interactome

Figure 5

Blocking integrin receptor αVβ6 reduces AAV9 transduction in multiple cell lines. (A) In U87 cells, pretreatment with 20 µg/ml of mouse anti-human αVβ6 integrin for 2 h reduces AAV9-GFP transduction as measured after 24 h compared to a PBS control. (B) Similar reductions in AAV9-GFP transduction are observed in HEK293T (n = 4), Hela (n = 4) and U87 (n = 3) cells that are pretreated with the αVβ6 integrin functional blocking antibody compared to PBS controls. Error bars represent mean ± SEM. **p < 0.01; ***p < 0.001; Scale bar 20 µm. Student’s unpaired t-test was used to compare the two groups. Hoechst 33342 counterstained nuclei stained white on a blue background.

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