Figure 8

Haemolytic activity of the two AfpB variants and PAF. Analyses were conducted in PBS (150 mM NaCl) (a), and in PBG (250 mM glucose) (b). Proteins were used at the concentrations indicated (from 1 to 100 µM). The cytolitic peptide melittin was included for comparison. The haemolytic activity is given as the mean ± s.d. of the percentage of human RBCs haemolysis (three replicates), as compared with the positive control in the presence of the detergent Triton X-100 (regarded as 100% haemolysis).