Figure 10

Far-UV CD spectra of HvASR1 (A) and TtASR1 (B) in the presence or absence of either 2.5 mM ZnSO₄, or 10% TFE or 10% TFE + 2 mM ZnSO₄ at 20 °C. Proteins were at 0.1 mg/mL in 10 mM sodium phosphate pH 7. Data is shown to the point at which the dyna voltage was in the permissible range. Data is representative of one out of two independent acquisitions. The insets show the α-helical content as derived as described in Methods (see Eq. 14). MRE: molar residue ellipticity.