Figure 1 | Scientific Reports

Figure 1

From: Combining membrane proteomics and computational three-way pathway analysis revealed signalling pathways preferentially regulated in human iPSCs and human ESCs

Figure 1

The workflow of our three-way pathway comparison on the comparative membrane proteomics of hiPSCs, hESCs, and HFF. (a) On the basis of spectral raw data, three profiles of protein expression log2 ratio, hiPSCs/HFF, hESCs/HFF, and hiPSCs/hESCs, were generated for hiPSCs versus HFF, hESCs versus HFF, and hiPSCs versus hESCs. (b) The similarity between hiPSCs and hESCs at protein expression level were evaluated by the Spearman correlation coefficient between hiPSCs/HFF and hESCs/HFF and a histogram describing the distribution of protein log2 ratios of hiPSCs/hESCs. (c) A three-way pathway comparison was performed on the significant pathways (obtained from GSEA) of hiPSCs/HFF, hESCs/HFF, and hiPSCs/hESCs to identify important pathways that may contribute to the differences between hiPSCs and hESCs during the reprogramming process of hiPSCs.

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