Figure 1
From: The NOD2 receptor is crucial for immune responses towards New World Leishmania species
NOD2 but not NOD1 plays an important role in monocyte-and lymphocyte-derived cytokines induced by after Leishmania pecies stimulation. Peripheral blood mononuclear cells (PBMCs, 5 × 105 cells/100 μL) from healthy individuals genotyped for NOD1 (Glu796Lys) and NOD2 (1007finsC) were stimulated with different stimuli including lysates of Leishmania spp. (50 μg/mL, L. (L.) amazonensis: L. amaz; L. (V.) braziliensis: L. braz), FK156 (10 μg/mL), MDP (10 μg/mL); Medium: non-stimulated cells. TNFα, IL-6, IL-1β and IL-8 concentrations were measured in supernatants by ELISA after 24 h of incubation. IFNγ and IL-17 were determined after 7 days of incubation: the NOD2 (A and B) and NOD1 (C) genotype. Bars represent individuals carrying no SNP (wild type, Wt, white bars), heterozygous SNP carries (He, black bars), or homozygous variation (Ho, grey bars). Data represent the mean ± SEM. *p < 0.05; Mann-Whitney U-test (A and B; Wt vs He); (C; Wt vs Ho).
