Figure 4
From: The NOD2 receptor is crucial for immune responses towards New World Leishmania species
NOD2 plays a role in control of Leishmania spp. infection. Monocyte-derived macrophages were obtained from PBMCs from wild type (wt) individuals (n = 4) carrying no mutations in NOD2 receptor (3020insC) (black circles) and individuals carrying mutations in NOD2 (n = 2, black squares) (A), after 5 days of differentiation. On day 5, macrophages (5 × 105 cells) were infected with promastigote forms of either L. (V.) braziliensis and L. (L.) amazonensis (2.5 × 105 parasites) during 4 h. (B) Macrophages from wt individuals (n = 4) were incubated in the absence (black balls - Vehicle DMSO) or presence (black squares) of Ponatinib (100 nM) 1 h before. Cells were washed out to remove the non-internalized parasites after 4 h, Ponatinib was added again and cells were incubated for 48 h. Coverslips were collected to determine macrophage infection index. Data represent individual values and horizontal lines represent medians. *p < 0.05; Wilcoxon test (DMSO vs Ponatinib).
