Figure 1
From: Brain activation induced by chronic psychosocial stress in mice
Timeline of the experiment and illustration of the chronic social defeat stress (CSDS) protocol. (a) Timeline of CSDS, manganese-enhanced magnetic resonance imaging and tissue collection. Each perpendicular line indicates one day. Estimated timeline for detection of Mn2+ by MEMRI and ∆FOSB by immunohistochemical staining is indicated by red and blue lines, respectively. (b) The experimental mouse in the defeated group (dark grey) was placed into the cage of a resident aggressor mouse (white) for a maximum of 10 minutes. It was then placed into the adjacent compartment of the same cage for 24 hours, retaining sensory contact without physical contact. The procedure was repeated for 10 days, each day in a novel resident aggressor mouse’s cage. (c) The control mouse was placed in a compartment adjacent to another control mouse, allowing sensory but not physical contact, for 24 hours. Each day the control mouse was placed into another cage with a novel control mouse, and the procedure was repeated for 10 days. MRI = magnetic resonance imaging.
