Figure 2

Analysis of differentially represented proteins. (a) Two-dimensional differential in-gel electrophoresis (2D DIGE) of hCFs exposed to cardiotoxic LCs was performed using four biological replicates. White and black labels indicate over-represented and under-represented species, respectively, identified in CardioLC-exposed hCFs compared to control or MMLC-exposed cells. (b) Volcano plots of the proteins identified by label-free shotgun analysis in each of the three pairwise comparisons. Three biological replicates per condition were analyzed, each one in three technical replicates. The proteins described in the manuscript (i.e. those with an adjusted p value < 0.01, identified with >2 distinct peptides and present in >75% replicates) are shown as colored dots and are indicated with their Uniprot accession number. Blue dots indicate under-represented proteins; red dots indicate over-represented proteins.