Figure 3
From: Establishment and phenotyping of disease model cells created by cell-resealing technique
Analysis of immunofluorescence images of HWT and HDb cells stained with antibodies against pAktS473 and Akt. (a) Schematic representation of the automated image acquisition system and the image analysis. (b) The mask of nucleus, cell, and resealed cells were determined by the binary images of Hoechst, Akt, fluorescein-dextran (FL-dex) staining. (c) HWT or HDb cells were treated with insulin for the indicated times and subjected to immunofluorescence using antibodies against pAktS473 and Akt. The feature quantities obtained from the images were subjected to principal component analysis. The individual HWT (blue) and HDb (pink) cells were plotted in a PC1-PC3 graph.
