Figure 4
From: Establishment and phenotyping of disease model cells created by cell-resealing technique
Quantification of the mean fluorescence intensity of pAktS473 and Akt, and the ratio of pAktS473 fluorescence to Akt fluorescence in HWT and HDb cells treated with a library of small chemical compounds. Serum-starved H4IIEC3 cells that had been grown on 96-well plates were permeabilized with SLO, and incubated with WT or Db liver cytosol that contained dextran conjugated with fluorescein. After resealing and subsequent incubation with DMEM(-FBS) for 1 hr in the presence of small chemical compounds, the cells were treated with insulin for 15 or 60 min and subjected to immunofluorescence using anti-pAktS473 and anti-Akt antibodies. The images were obtained by using the automated image acquisition system. The mean fluorescence intensities of pAktS473 and Akt, and the mean ratio of pAktS473 fluorescence to Akt fluorescence are shown in the graph.
